Coding

Part:BBa_K2286011

Designed by: Licong He   Group: iGEM17_UESTC-China   (2017-10-20)


HheC-F12Q-F186L-W249P: halohydrin dehalogenase site-directed mutant

This is the site-directed mutant of halohydrin dehalogenase. Based on the excellent mutant HheC-W249P, We change the phenylalanine to glutamine in the 12 site and phenylalanine to leucine in the 186 site. And this sequence is codon-optimized for E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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